mir-143 induces expression of aim2 and asc in jurkat cell line
نویسندگان
چکیده
background: absent in melanoma 2 (aim2) is an intracellular microbial dsdna sensor which plays an important role in production of proinflammatory cytokines through apoptosis associated speck-like protein containing a caspase activation and recruitment domain (asc) and caspase-1. micro-rnas (mirnas) play important roles in regulation of immune related genes. however, there is little information regarding the effects of mirnas on the aim2 and asc expression. objective: to determine the mrna levels of aim2 and asc in jurkat cell line following introducing mirna-143 (mir-143). methods: mir-143, a scrambled sequence and pbs were introduced separately, to the jurkat cell lines and the mrna levels of aim2 and asc were examined in parallel with beta-actin and gapdh (as housekeeping genes) using real-time pcr technique. results: the mrna levels of aim2 and asc were significantly increased in the mir-143 transfected jurkat cells when compared to the scrambled sequence or pbs treated cells. conclusions: mir-143 can lead to increased expression of aim2 and asc mrnas. considering the significance of aim2 and asc in dna sensing and inflammosome formation, it can be considered as a therapeutic agent for the treatment of chronic infectious diseases, especially viral infections.
منابع مشابه
MiR-143 Induces Expression of AIM2 and ASC in Jurkat Cell Line
Background: Absent in Melanoma 2 (AIM2) is an intracellular microbial dsDNA sensor which plays an important role in production of proinflammatory cytokines through Apoptosis associated Speck-like protein containing a Caspase activation and recruitment domain (ASC) and Caspase-1. Micro-RNAs (miRNAs) play important roles in regulation of immune related genes. However, there is little information ...
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عنوان ژورنال:
iranian journal of immunologyجلد ۱۰، شماره ۲، صفحات ۱۰۳-۱۰۹
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